Autonomous mRNA Vaccine Design Agent โ Dr. Antonino Parisi PhD
โ ACTIVE Nicotiana benthamiana ยท GFP Fluorescence Protocol
In vitro transcription of GFP mRNA with 5' Cap and poly-A tail. Pseudouridine substitution for stability. Expected yield: 50-100 ฮผg.
Encapsulate mRNA in LNPs (ionizable lipid:DSPC:cholesterol:PEG-lipid 50:10:38.5:1.5). Size target: 80-120 nm. Encapsulation efficiency >80%.
Needleless syringe infiltration into abaxial leaf surface of 4-6 week old N. benthamiana plants. Concentration: 0.1-1 ฮผg/ฮผL LNP-mRNA solution.
UV light (365 nm) illumination of infiltrated leaves. GFP fluorescence visible as bright green signal. Photograph with UV filter.
Quantify fluorescence intensity. Generate PDF report with images and data. Upload to ParisiVaxAI dashboard.
mRNA Synthesis: ~$300 | LNP Formulation: ~$200 | Plant Work: ~$150 | Total: ~$650 USD
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